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Posts by miller.time.716
Comment: C: Question on RNA-seq
... 1. Sorry, forgot to mention these are human neural stem cell lines. 2. So I assume you mean that I should run each replicate separately instead of combining into a single tube? 3. Without ribo-depletion, wouldn't polyA enrichment exclude rRNA? ...
... I'm looking to perform RNA-seq on pairs of cell lines to look at differential gene expression and wondering what I can get away with to lower costs. 2 questions: 1) How many reads would be a good starting point? Would 20M be a reasonable place to start? 2) I understand I should have at least 2 bi ...
... I have 2 cell lines which show a difference in growth advantage when implanted in immunocompromised mice. If I want to identify differences in gene expression between the 2 cell lines, how many replicates of each line would I need and how much read depth should I aim for? The cell lines are human ...
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