User: wizofe

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wizofe0
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@wizofe
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Posts by wizofe

<prev • 5 results • page 1 of 1 • next >
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bowtie2 alignment with ambiguous bases
... Hi all! My reads contain several ambiguous (N) bases at both the 5'-end and 3'-end (specifically 5 and 4 bases respectively). So it looks like this: $ head trimmed_Negative.fq @AFPN02.1_merge-1076320 NNNNNAAGGTCGCTAATCTCTTTACGCAGATTTTTTATTCCTTCAACTAACAGCGNNNN + #####BACCCGFG ...
bowtie2 N ambiguous written 8 months ago by wizofe0 • updated 8 months ago by genomax75k
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Comment: C: bio-newbie / gene research / tools and methods
... That make sense :) I agree. The point is exactly that without assessing the biological question it doesn't make any sense to start using tools and doing alignments or BLAST'ing! Happily enough, I just discovered in my Uni library the book Exploring Bioinformatics by Clair & Visick [2010], whi ...
written 23 months ago by wizofe0
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Comment: C: bio-newbie / gene research / tools and methods
... @jrj.healey: I appreciate your help, I am trying to setup a Blast+, Artemis and UNIPROT workflow locally. I just feel really lacking the biology background and I am on my baby bioinformatics steps and having all those lectures and courseworks that are super stressfull (mind, I am doing a part-time M ...
written 23 months ago by wizofe0
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bio-newbie / gene research / tools and methods
... hello, world! Newbie in bioinformatics, with an assigned project but not enough background and course information to be able to go forward. I would be more than happy if somebody could guide me on the following (its a research on a prokaryotic gene/protein). > Conservation of this gene/protein ...
gene bioinformatics methods research written 23 months ago by wizofe0 • updated 22 months ago by Biostar ♦♦ 20
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research on gene, which tools can i use?
... Hello stars! I have an assignment on my degree with the description of "providing information on the rpfB gene in the bacterium Mycobacterium tuberculosis". Unfortunately there is not enough information on the tools to be used for gathering this information but there are some hints about the topic ...
gene blast written 24 months ago by wizofe0

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