User: ovariohisterectomia

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Posts by ovariohisterectomia

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NCBI Primer-BLAST primers
... hi everyone. ill be brief, does anyone know if when i use this tool [https://www.ncbi.nlm.nih.gov/tools/primer-blast/][1] the given primers are "ready to use" or if i have to "convert them" because this tools send me the reverse, complement, or reverse-complement sequence of the primer? Thank you ...
primers pcr written 3 months ago by ovariohisterectomia20 • updated 3 months ago by Scott McKay20
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Comment: C: Choosing Hub genes inside a module (WCGNA - COEX NETWORK)
... Thank you Kevin! I will see iT. By the way, what is your opinion about criteria of kME ≥0.85 and the top 10% of the gene list inside the module? Thank you! ...
written 4 months ago by ovariohisterectomia20
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Choosing Hub genes inside a module (WCGNA - COEX NETWORK)
... Hi everyone. O Will be brief How You choose the hub genes in a Signed coex network? I have seen Many posts here, and i have found: A choosing kME over 0.75 I have My own list with kME of My modules B Using cytoscape cytohubba For me , did not work in the 3.8 version of cytoscape C Using a com ...
rna-seq wcgna written 4 months ago by ovariohisterectomia20 • updated 4 months ago by Kevin Blighe70k
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Comment: C: WCGNA to Cytoscape
... PART II Head of annot (this is the annotation file that i have changed . it is a new file, not a modifed one and the source is here [https://www.ncbi.nlm.nih.gov/genome/browse/#!/proteins/10706/50662%7CEchinococcus%20granulosus/][2] ) head(annot) X.Name Accession Start Stop Stra ...
written 5 months ago by ovariohisterectomia20
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Comment: A: WCGNA to Cytoscape
... hi again Kevin, and thank you for your patiente. I will tell you what i have done now. 1st of all my annotation file, was wrong, so i changed it (in the command line says the same name that before, but it is just a "practical" way, the file now is differente). I update what i do (and tell you too w ...
written 5 months ago by ovariohisterectomia20
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Comment: C: WCGNA to Cytoscape
... Hi Kevin! Thank You for your help. Still not solved, because modGenes seems to be an empty file... thanks! ...
written 5 months ago by ovariohisterectomia20
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Comment: A: WCGNA to Cytoscape
... hi everyone, i changed my script like this: module = "black" probes = names(datExprhigado) annot = read.csv(file = "anotacion.csv") TOM = TOMsimilarityFromExpr(datExprhigado, power = 18) inModule = is.finite(match(moduleColorsHigado, module)) modProbes = probes[inModule] ...
written 5 months ago by ovariohisterectomia20
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Comment: C: WCGNA to Cytoscape
... Thank you kevin. Here says : str(modProbes) chr [1:306] "EGR_10759" "EGR_10652" "EGR_10430" "EGR_10293" "EGR_10234" ... > str(modTOM) num [1:306, 1:306] 1 0.1531 0.0905 0.0385 0.0181 ... - attr(*, "dimnames")=List of 2 ..$ : chr [1:306] "EGR_10759" "EGR_10652" "EGR_1 ...
written 5 months ago by ovariohisterectomia20
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Comment: C: Cytoscape GO Enrichment Annotation file trouble (BiNGO)
... Hi Scooter and sorry for my late answer. I have seen STRING and ClueGO and there is no E granulosus information. I have used gProfiler already, for each group of genes. I was trying to use BiNGO cause some guys that worked with E granulosus told me that they worked with BiNGO... but i do not know ...
written 5 months ago by ovariohisterectomia20
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Cytoscape GO Enrichment Annotation file trouble (BiNGO)
... Hi everyone and thank you in advance. I Have a list of genes that i got from a module from WCGNA coex network. Those are the first lines of my list EGR_11308 EGR_11303 EGR_11301 EGR_11292 EGR_11271 EGR_11270 EGR_11255 EGR_11254 EGR_11197 EGR_11194 There ar ...
cytoscape go analysis rna-seq written 6 months ago by ovariohisterectomia20 • updated 6 months ago by scooter420

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Popular Question 4 months ago, created a question with more than 1,000 views. For Error in -delete: invalid argument to unary operator
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