User: annsun89

gravatar for annsun89
annsun8910
Reputation:
10
Status:
New User
Location:
Last seen:
2 weeks ago
Joined:
9 months ago
Email:
a*******@gmail.com

Posts by annsun89

<prev • 3 results • page 1 of 1 • next >
1
vote
1
answer
252
views
1
answers
Answer: A: Trimmomatic adapter clipping removes many reads even if "keepBothReads" paramete
... Hi, I read the manual and is the name of the parameter but it's not how to set it. Instead, try java -jar trimmomatic-0.33.jar PE R1.fastq.gz R2.fastq.gz PR1.fastq.gz UPR1.fastq.gz PR2.fastq.gz UPR2.fastq.gz ILLUMINACLIP:TruSeq3-PE-2.fa:2:30:10:1:TRUE ...
written 10 weeks ago by annsun8910
0
votes
0
answers
200
views
0
answers
Comment: C: Leftover adapter sequences after Trimmomatic trimming?
... Ah, thank you very much, both for the answer and for the extra help! I will try bbduk.sh and see how it goes. I used trimmomatic because it was a tool already available on the cloud cluster I'm using (compute canada). However, is there a specific reason to use bbduk.sh instead of trimmomatic other ...
written 10 weeks ago by annsun8910
1
vote
0
answers
200
views
0
answers
Leftover adapter sequences after Trimmomatic trimming?
... Hi everyone, I'm a wet lab biologist who now has 100+ RNA-seq samples to analyze so it's been a steep learning curve. Any help would be super appreciated! I have PE 125bp fastq files from an Illumina Hiseq and my fastqc analysis shows Illumina Universal Adapter contamination. I used trimmomatic t ...
trimmomatic adapters trimming rna-seq written 10 weeks ago by annsun8910 • updated 10 weeks ago by genomax55k

Latest awards to annsun89

Scholar 10 weeks ago, created an answer that has been accepted. For A: Trimmomatic adapter clipping removes many reads even if "keepBothReads" paramete

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1678 users visited in the last hour