User: benbio

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benbio10
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Posts by benbio

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Comment: C: Any reason for higher reported levels of non-CG methylation when analysing non-d
... Thanks for your suggestions, Mark. Point 3 is the kind of thing I was thinking might be the reason, as CpG context is unaffected, reported at similar levels in both directional and non-directional libraries - I assume such issues as 1 and 2 would affect all contexts. I'll try your suggestion of usin ...
written 12 months ago by benbio10
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Any reason for higher reported levels of non-CG methylation when analysing non-directional WGBS libraries with Bismark?
... Hi, just FYI I've also posted a variation of this question on [seqanswers][1], I'll update both posts accordingly with any responses. ----- I've been analysing some WGBS libraries for a couple of different insect species, using Bismark for alignment. For context in most insects methylation levels ...
bisulfite bs-seq bismark sequencing written 12 months ago by benbio10
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Bisulfite sequencing data - an issue with specific bases in specific tiles
... Hi, I'm working with a set of whole genome bisulfite sequencing data that has some quality issues I'm struggling to resolve satisfactorily. I was wondering if anyone has experienced something similiar and might be able to offer some advice. It's HiSeq X, 150bp paired end reads, an insect species ( ...
qc methylation bs-seq bisulfite written 2.6 years ago by benbio10

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