User: M.Nadeem Khan

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Posts by M.Nadeem Khan

<prev • 11 results • page 1 of 2 • next >
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Comment: C: assembly of contigs into scaffolds
... Dear thank you for your response. these are 1500 short reads. I want to process these reads. I just want to annotate these ...
written 6 weeks ago by M.Nadeem Khan0
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assembly of contigs into scaffolds
... Hello All.. I sequenced a fungal genome, assembled it into reads and then into contigs, then I tried to annotate it through RAST but it did not proceed and the command was "your sequence has more contigs and cannot be processed" the number of contigs is exceeding 1000. the size is 23MB. Is there any ...
assembly contigs scaffolds written 6 weeks ago by M.Nadeem Khan0 • updated 6 weeks ago by shubhra.bhattacharya120
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primers for whole metagenome
... Hello All I want to do amplify all the bacterail genomic contents present in environmental samples. What kind of primers I have to use? Primers used for 16s are enough for this procedure? or I have to desgin special type of primers? ...
genome sequencing written 4 months ago by M.Nadeem Khan0 • updated 10 weeks ago by Fabio Marroni2.0k
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Comment: C: Computational tools and softwares
... Thank you very much dear ...
written 4 months ago by M.Nadeem Khan0
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Comment: C: Computational tools and softwares
... Thank you dear karthic, yes you are right mostly there is a solution to the issues. I am doing a project "Pan-genome analysis of lactic acid bacteria". The Higher Education Commision of my country is paying for the said project and they have given me 162 USD for software purchasing for the current m ...
written 4 months ago by M.Nadeem Khan0
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Computational tools and softwares
... Dear All Hope you all are fine and good. I am new to the computational world but I have to use this till completion of my Ph.D. project. I have to work on the structural and functional genomics of prokaryotes. I have a specified budget to purchase the software. Now its the beginning and I am unfami ...
genome alignment sequencing written 4 months ago by M.Nadeem Khan0 • updated 4 months ago by karthic100
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Comment: C: Conversion of Fasta file
... Dear Sej Modha.. I want to submit my data to the SRA portal and it accepts only FASTQ. I have scores but cannot add these ...
written 7 months ago by M.Nadeem Khan0
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Comment: C: Conversion of Fasta file
... I know this. thanks for trying to help me out. ...
written 7 months ago by M.Nadeem Khan0
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Comment: C: Conversion of Fasta file
... Dear Carlo Yague Thanks for your reply. I have the QAUL file too but as I mentioned early, am a beginner to the computational biology. I don't know how to convert. Is there any tool which can do this? I tried online tools but failed. or is there any such sequence which I could use as a model and do ...
written 7 months ago by M.Nadeem Khan0
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Conversion of Fasta file
... Hello Folks!! Hope all of you are fine, doing well and everything around you is going well. I have a FASTA file of about 1GB that contains 16s sequences of different bacterial strains. I want to submit my data to NCBI where the only FASTQ format is accepted. I want to change the format but i don't k ...
genome sequence tool sequencing written 7 months ago by M.Nadeem Khan0 • updated 7 months ago by RamRS20k

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