User: evakoole

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evakoole0
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Posts by evakoole

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Comment: C: Low input ChIP-seq on frozen tissue
... Okay thank you! When I find out more, I'll put it under this message but it will take a while before my experiments are done. ...
written 6 months ago by evakoole0
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Comment: C: Low input ChIP-seq on frozen tissue
... Hi Jotan, thank you for your reply! For me, the biggest concern is keeping the nuclei intact until after the fixation step. To create better access for the formaldehyde, the tissue needs to be disaggregated (dounce homogeniser for example) If the nuclei are damaged, the DNA:protein interactions can ...
written 6 months ago by evakoole0
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Low input ChIP-seq on frozen tissue
... Dear all, Currently I am working on a project to find the most optimal protocol for doing ChIP-seq on frozen tissues. Most protocols are designed for cell lines, however, ChIP-seq in vivo (small biopsies for example) is really interesting to me. Especially differences in transcription factors and h ...
chip-seq written 6 months ago by evakoole0

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