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Posts by bioinfo_wen
... There are 2 replicates sample in my RIP-seq for each condition, but another sample performed better than this. I maped my reads to mm10 with hisat2, the overall mapping rate about 69%,and nearly 50% of total reads aligned concordantly exactly 1 time. sequencer was illumina Hiseq 3000, And I am wonde ...
... my mouse RIP-seq data have abnormal GC content and the fastqc result in 'GC content' have 2 peaks, first one is about 55% in x axis, y axis is about 500000. the second one as about 85% in axis,y axis is about 1600000, very sharp peak.besides, the GC content each base is not about 25% for A,T,G,C. th ...
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