User: teleos27

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teleos270
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Posts by teleos27

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Comment: C: How to make the Reverse sequence from single forward sequences Illumina Hiseq?
... Could you help me with a link with this information. I'm very confused I found this information: https://stackoverflow.com/questions/32485654/what-do-illumina-hiseq-miseq-paired-end-reads-look-like http://thegenomefactory.blogspot.de/2013/08/paired-end-read-confusion-library.html ...
written 5 weeks ago by teleos270
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Comment: C: How to make the Reverse sequence from single forward sequences Illumina Hiseq?
... Thanks for your response. I was confused with the R2 output in pair end. I thought it was reverse complement but that is incorrect it's only the reverse. If I do the reverse of my fastq sequences for my single read data, I think that would work, or not? ...
written 5 weeks ago by teleos270
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How to make the Reverse sequence from single forward sequences Illumina Hiseq?
... Hello, I am working with Illumina hiseq 2000, only single reads. I got my sequences from the EBI database:https://www.ebi.ac.uk/ena/data/view/PRJEB15445 I'm planning on doing some further analysis and for that I need pair-end sequences. I need to artificially generate the reverse reading. I am bi ...
forum written 5 weeks ago by teleos270
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Comment: C: Reverse complement for multiple fastq files
... Yes I know :( Thank you so much! ...
written 5 weeks ago by teleos270
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Comment: C: Reverse complement for multiple fastq files
... Thanks for your suggestion I'll take a look at this. ...
written 5 weeks ago by teleos270
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Comment: C: Reverse complement for multiple fastq files
... Thanks for your response! I am so sorry for this, but I have a different error for something else. I really appreciate your thoughts on this. I now get this error: Traceback (most recent call last): File "./reverse_comp.py", line 9, in SeqIO.write(records,seq_record.id + "R2.fastq",' ...
written 5 weeks ago by teleos270
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Comment: C: Reverse complement for multiple fastq files
... Thanks so much for your response! I replaced my file name with sys.argv[1] and import sys, and saved the script. Then I created a bash script: #!/bin/bash for i in files;do ./reverse_comp.py done I run the script and I get the following error: Traceback (most recent call ...
written 6 weeks ago by teleos270 • updated 6 weeks ago by genomax46k
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Reverse complement for multiple fastq files
... Hello! I have a data set of various fastq files, all of these are forward readings: ERS1355434.fq ERS1355435.fq ERS1355436.fq ERS1355437.fq ERS1355439.fq ERS1355440.fq ERS1355442.fq I want to get the reverse complement for these fastq sequences. I am using biopython to do this, so for one fastq ...
biopython written 6 weeks ago by teleos270 • updated 6 weeks ago by genomax46k

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