User: smallfish

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smallfish10
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Posts by smallfish

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How to process the raw reads from miRNA-seq
... Hi, Many studies reported the peak length of miRNA of 22 nt in fishes. However, I obtained the peak length of 24 nt. Is there any mistakes in the data processing? The raw reads were obtained by illumina hiseq xten sequencing, and were processed with the program cutadapt to trim the adaptor sequenc ...
rna-seq written 2.3 years ago by smallfish10 • updated 2.3 years ago by k.kathirvel93260
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How to perform KaKs_Calculator from SNPEff's output
... Hi, all, I have obtained the annotated SNP (VCF file) by SNPEff. How to calculate ka/ks using KaKs_Calculator or other programs. ...
rna-seq written 2.5 years ago by smallfish10
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Comment: C: How to know synonymous and non-synonymous SNPs from transcriptome sequencing
... Thanks very much. It's a good idea. ...
written 2.7 years ago by smallfish10
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How to know synonymous and non-synonymous SNPs from transcriptome sequencing
... I obtained a SNP list (VCF format) from a sample's transcriptome (assembled by Trinity). ORFs were identified using TransDecoder. How I classify these SNPs? How to calculate ka/ks? ...
rna-seq snp written 2.7 years ago by smallfish10 • updated 2.1 years ago by jfo0

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