User: inah

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inah20
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Posts by inah

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Comment: C: miRNA-seq Trimmomatic adapter file
... To all who responded, thanks, I have tried to do what you suggested. Until now I have been using ea-utils mcf for adapter trimming and btrim for mild quality trimming (on mRNA, miRNA and total RNA). For mcf, I pass on an adapter fasta file provided by Illumina shown below. When I pass this file to ...
written 12 weeks ago by inah20 • updated 12 weeks ago by genomax57k
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miRNA-seq Trimmomatic adapter file
... Hi, what adapter file do I need to use to run Trimmomatic on miRNA-seq data generated on an Illumina Next-seq? I don't see any miRNA adapter file provided with Trimmomatic (or am I missing it), do I need to create one and how? Thanks, Ina ...
trimmomatic adapter mirna-seq written 12 weeks ago by inah20 • updated 12 weeks ago by WouterDeCoster32k
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counting after aligning miRNA reads to miRBase mature miRNA instead of reference genome
... I have human miRNA-seq data. In the past I have aligned these data using Bowtie2 to the reference genome (Homo_sapiens.GRCh38.dna.toplevel.fa), and I have subsequently performed counting using featureCounts with the annotation file hsa.gff3 from miRBase. . Now I have aligned the reads to the mature ...
mirna-seq written 6 months ago by inah20 • updated 5 months ago by toralmanvar650

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