User: younglin113

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younglin11350
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Posts by younglin113

<prev • 26 results • page 2 of 3 • next >
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Comment: C: low unique mapping ratio of RNA-seq data
... Actually, many papers show that they will use unique mapping reads for downstream analysis. And once I saw a writer said we could use the threshold of Q>20 to filter the unique mapping reads. That how my results came. ...
written 13 months ago by younglin11350
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Comment: C: low unique mapping ratio of RNA-seq data
... Thanks a lot for your help. I did define the fraction of rRNA by mapping all clean reads to rRNA sequences. ...
written 13 months ago by younglin11350
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Comment: C: low unique mapping ratio of RNA-seq data
... ![enter image description here][1] [1]: https://i.ibb.co/c8CJrMr/image.png ...
written 13 months ago by younglin11350
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Comment: C: low unique mapping ratio of RNA-seq data
... The FastQC result seemed fine, isn't it? ...
written 13 months ago by younglin11350
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Comment: C: low unique mapping ratio of RNA-seq data
... yes, I only have 4 samples. And the hiast2 command is : `hisat2 -p 16 -x $hisat_index -1 $R1_fq -2 $R2_fq -S $out_sam` ...
written 13 months ago by younglin11350
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Comment: C: low unique mapping ratio of RNA-seq data
... Of course, I used the Trimmomatic for cleaning, and Hisat2 for alignment, and then use command `samtools view -q 20 ` to filter the unique mapped reads. ...
written 13 months ago by younglin11350
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Comment: C: low unique mapping ratio of RNA-seq data
... ![enter image description here][1] [1]: https://i.ibb.co/hK9Ccdf/image.png ...
written 13 months ago by younglin11350
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low unique mapping ratio of RNA-seq data
... I've been confused of my recent RNA-seq analysis result. Here is the situation: the RNA-seq results were obtained by Illumina pair-end 150bp sequencing, after trimming the adapters and low-quality bases, the clean reads were mapped to GRch38 genome, but the results showed that the unique mapping rat ...
next-gen rna-seq written 13 months ago by younglin11350 • updated 13 months ago by bruce.moran730
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Comment: C: I don't know how to make this bioinformatic picture
... It really helps a lot, thanks for your help! ...
written 23 months ago by younglin11350
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Comment: C: I don't know how to make this bioinformatic picture
... Thanks a lot for your answer!! ...
written 23 months ago by younglin11350

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Popular Question 11 months ago, created a question with more than 1,000 views. For I got some diffculties when run rMATs

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