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questions
1
vote
2
replies
515
views
16S amplicon sequencing input data (spreadsheet): barcode vs. linker primer seqs?
barcode
amplicon
16s
4 months ago by
sovrappensiero
▴ 90
17
votes
8
replies
1.5k
views
Where do find virulence gene information in a gff/gtf file?
microbial
genomics
gff
annotation
gtf
13 months ago by
sovrappensiero
▴ 90
5
votes
5
replies
876
views
Simulating metatranscriptomic shotgun reads
metatranscriptomic
RNA
simulated-reads
shotgun-sequencing
updated 14 months ago by
Mensur Dlakic
★ 27k • written 14 months ago by
sovrappensiero
▴ 90
2
votes
3
replies
730
views
biological vs. technical definition of "insert size"
grinder
simulated-reads
shotgun-sequencing
updated 14 months ago by
ATpoint
82k • written 14 months ago by
sovrappensiero
▴ 90
0
votes
4
replies
931
views
Alternative to NCBI nt for MEGAN-LR?
taxonomy
database
pacbio
index
smrt
updated 2.0 years ago by
dportik
• 0 • written 2.0 years ago by
sovrappensiero
▴ 90
6
votes
7
replies
3.0k
views
kallisto "gene" counts
gtf
rna-seq
kallisto
updated 22 months ago by
Ram
43k • written 2.0 years ago by
sovrappensiero
▴ 90
5
votes
12
replies
3.0k
views
Secrets to finding metadata on SRA data?
metadata
sra
ncbi
biosample
bioproject
updated 2.1 years ago by
Jeremy Leipzig
22k • written 2.1 years ago by
sovrappensiero
▴ 90
0
votes
0
replies
614
views
NCBI EUtils: identifying projects with both NGS and Sanger sequencing
ncbi
eutils
data type
3.5 years ago by
sovrappensiero
▴ 90
5
votes
11
replies
2.2k
views
Elink to get PMID associated with a BioProject or SRA entry
eutils
ncbi
bioproject
pmid
pubmed
updated 3.5 years ago by
Istvan Albert
100k • written 3.5 years ago by
sovrappensiero
▴ 90
1
vote
3
replies
1.2k
views
primersearch Illegal character '/' error: EMBOSS An error in primersearch.c at line 499
primersearch
emboss
error
warning
4.4 years ago by
sovrappensiero
▴ 90
2
votes
7
replies
1.8k
views
Entrez Direct E-utilities on command line: efilter parameters for limiting efetch results
ncbi
efilter
efetch
elink
biosample
updated 4.4 years ago by
GenoMax
141k • written 4.4 years ago by
sovrappensiero
▴ 90
5
votes
4
replies
9.8k
views
Normalization scaling factors: formula for applying them to raw counts
normalization
edgeR
DESeq2
R
5.9 years ago by
sovrappensiero
▴ 90
0
votes
8
replies
2.4k
views
Normalization amplicon data using mean depth and coverage
normalization
amplicon
depth
coverage
5.9 years ago by
sovrappensiero
▴ 90
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