User: daisy.ko
daisy.ko • 20
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Posts by daisy.ko
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... I am new will dealing with the SSMethylSeq data and when I want to further analyse a sample, I found that different from other samples which the sequenced fragments were targeted at the bottom strand, that sample have a quite large number of reads from the top strand. Just shown as below:
Numbe ...
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... Hi Felix,
I think that I should post it here ...
Thanks for answering. So in that case, I should use the default directional setting with Bismark. But however, I found that even using this setting would get an extreme difference in the read of OB and OT. How can coverage be calculated when we are ...
written 5 months ago by
daisy.ko • 20
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... Seems that we are having the same problem .... Not sure whether it is because of data quality ... Do you also have a larger number of aligned read in the bottom strand? ...
written 5 months ago by
daisy.ko • 20
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... I have a paired-end data from "sureselect methyl-seq target enrichment system" which is a kind of Bisulfite sequencing data produced in a similar manner as RRBS as far as I understand.
However, after aligning with Bismark with the default Bowtie2, I found that there is a really large bias for the r ...
written 5 months ago by
daisy.ko • 20
• updated
5 months ago by
Felix Krueger • 40
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