User: Peter

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Peter0
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Posts by Peter

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Comment: C: rRNA remove from RNAseq data
... thank you! I only see the rRNA rate in my fastq file using BWA for mapping to refseq rRNA sequences. I just want to know the efficiency of the kit used. I don't know why 45S pre-rRNA is not removed. 20% accounts for about 6G of data, which I think is a big waste. I want to reduce this waste. I am ...
written 16 months ago by Peter0
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rRNA remove from RNAseq data
... Hi everyone, I mapped human RNAseq raw data to Refseq rRNA sequences using bwa, it turned out that 20% raw reads mapped to rRNA sequences. Is it normal? But I have used a kit to remove rRNA including cytoplasmic(5s, 5.8s, 18s, 28s) and mitochondrial(12s, 16s) ribosomal RNA. I found that the most m ...
rna-seq written 16 months ago by Peter0 • updated 16 months ago by ATpoint25k
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Comment: C: How to determine biallelic CDK12 mutations?
... OK! Thank you for your help! ...
written 16 months ago by Peter0
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How to determine biallelic CDK12 mutations?
... Recently I started the concerning mutations in CEBPA gene within patients with prostate cancer. I got two heterozygous for p.D52fs / p.S323* mutations from my wes data. Could anyone tell me how to clarify whether the different mutations were on the same allele or on different alleles? ...
genome written 16 months ago by Peter0 • updated 14 months ago by Biostar ♦♦ 20

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