User: lugonauta
lugonauta • 10
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- 11 months, 2 weeks ago
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Posts by lugonauta
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... Hello,
I´m trying to annotate a set of phage genomes. I firstly used PHANOTATE in order to predict ORFs. I obtained a .gb file. I want to use this .gb file to predict the functionality of the hypothetical ORFs. I tried to use RASTtk, but I don´t know how to prevent the pipeline from predict ORFs aga ...
written 6 weeks ago by
lugonauta • 10
• updated
6 weeks ago by
WouterDeCoster ♦ 39k
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... Hi friends!
I'm interested in perfoming an analysis of the **fungal community** from a set of faecal samples. I have some skills about 16S rDNA amplicon approach for Microbiome analysis, using QIIME2, but I'm completely neofyte in the Mycobiome world.
Could anyone explain me how to perform such kind ...
written 6 months ago by
lugonauta • 10
• updated
6 months ago by
ashishbansal12300 • 0
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... OK, I will try to exclude small contigs mapping it against a reference genome (using mauves). Anyway, would you recommend me any software to re-assemble contigs into scaffolds? Thanks ...
written 9 months ago by
lugonauta • 10
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... Hi everyone, I'm new in WGS, in fact it is my first time! I´m trying to sequence a Lactobacillus fermentum strain. I would like to deposit it on GenBank, so I need to obtain a robust and reliable final product, but it is not necessary to close the genome (maybe a draft genome composed by 100-200 con ...
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Comment:
C: Buy PC for metagenomics
... Hi, sorry about not be as concrete as I should. Our goal is to support the needs of our clinical microbiology laboratory, therefore we would not have a constant activity on a large scale. None of us have broad experience in bioinformatics nor NGS (we about to finish the residency training program in ...
written 11 months ago by
lugonauta • 10
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... Hi everyone,
I'm new in bioinformatics. I'm working in a clinical microbiology laboratory, and my chief wants to stablish a sequencing department for clinical and research purposes (whole genome sequencing for studying outbreaks, 16s metagenomics for studying microbiota, etc). We want to buy a des ...
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