User: arpankbasak

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arpankbasak10
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Posts by arpankbasak

<prev • 13 results • page 1 of 2 • next >
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Comment: C: How to design degenerative primer in R?
... Thanks for the suggestion, this one did the trick. AMAZING! ...
written 9 weeks ago by arpankbasak10
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Comment: C: Is it me or everyone, DECIPHER package holds good for GenBank format, especially
... Well, its pretty complicated even when you convert it, (I tried it). Whats behind SQL is a "Black Box". But I waiting for the next update. Probably they'll fix these issues, with AAstringsset objects. The simple way will be to take the data into .txt format. But still, the deeper operations are bec ...
written 4 months ago by arpankbasak10
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Comment: C: Is it me or everyone, DECIPHER package holds good for GenBank format, especially
... In short I want reverse translate my peptides such that I can fetch possible exons from genome coordinates coding for my peptides, more like de-novo method. BY THE WAY/S, DECIPHER is quite popular package or pipe, you can do almost everything with it and its really fast and not at all furious on RA ...
written 4 months ago by arpankbasak10
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Is it me or everyone, DECIPHER package holds good for GenBank format, especially for nucleotides.
... Hi, I'm struggling here working with amino acid sequences and DECIPHER package. I figured DECIPHER works smoothly when the format is GenBank. Which can be challenging to download manually for these number of sequences. Some of my peptides are unidentified. Is anyone aware of the usage and flexibilit ...
decipher R written 4 months ago by arpankbasak10
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Comment: C: How to characterise a protein "enzyme", and find evolutionary divergence into tw
... Thanks, this article provides a good start. ...
written 4 months ago by arpankbasak10
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Comment: C: How to characterise a protein "enzyme", and find evolutionary divergence into tw
... No, I meant simple steps like steps represented with ">" or "|" Example: Dna_Isolation > Quantification > PCR > Library_preparation ... ...
written 4 months ago by arpankbasak10
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How to characterise a protein "enzyme", and find evolutionary divergence into two distinct pathways?
... Hi, I was wondering if any one can tell me, how I can characterise a protein divergence evolution significant in metabolic pathway. I'll be using rDNA technology to express the possible genes of these "proteins" or "enzymes" to be more specific to express them in a vector and then infect them on pla ...
pathway proteins enzymes protocol written 4 months ago by arpankbasak10
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Comment: C: How to compute convergence in phylogeny in R?
... Thanks..!! I'll go through it. ...
written 4 months ago by arpankbasak10
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Comment: C: How to compute convergence in phylogeny in R?
... Firstly, queries are aa seqs (Enzyme to be more specific), so the variation in the active site will be my marker. If you could send me the git_link of your package I'll try my luck. DECIPHER is an independent package, you can do almost everything with it, but it is not that versatile when it comes t ...
written 4 months ago by arpankbasak10
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Comment: C: How to design degenerative primer in R?
... I meant DECIPHER.codes by E. Wright. With this package I have successfully designed fwd and rev primers (barcoded) but I havent tried degen-primers, can it be done..? I couldn't find the option for degen-primer. ...
written 4 months ago by arpankbasak10

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