User: dasakan

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dasakan0
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Posts by dasakan

<prev • 6 results • page 1 of 1 • next >
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Comment: C: Extracting alignment coordinates from SAM file
... Hi, The meaning goes like this: Contig: assembled sequences of RNA-seq reads, where each read is considered as transcript. As these sequences were generated from RNA sequences, so there will not be intronic sequences. Hence, if we align a contig sequence (mRNA/DNA) to genomic sequence, it may alig ...
written 6 months ago by dasakan0 • updated 6 months ago by finswimmer12k
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Extracting alignment coordinates from SAM file
... Hi, I have a SAM alignment file. Alignment was done using BWA MEM between reference genome (in scaffolds, not a finished genome) and assembled transcripts (as contigs). I am interested to extract the genome coordinates of each contig/split contigs (as these are transcripts, so while aligning to the ...
assembly alignment sequencing written 6 months ago by dasakan0
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Comment: C: Aligning transcript with known position for SNP to draft genome to find the posi
... Thanks:) will try that way.. ...
written 7 months ago by dasakan0
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Aligning transcript with known position for SNP to draft genome to find the position of SNPs
... Hi, I have assembled transcriptome (sequenced by RNAseq) with known position of SNPs (Vcf file is there). In the mean time, I have received the draft genome in different scaffolods. To design probe to amplify the SNP target, I am interested to use the genome sequences, however, I donot know the SNP ...
genome alignment snp written 7 months ago by dasakan0 • updated 7 months ago by Vitis2.2k
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(Closed) how to exit virtualenv in mac terminal
... How to exit/leave from the python virtual environment (as mentioned in the following) to the real workon machine (in mac terminal). I tried deactivate and source deactivate, its not working. Last login: Thu Sep 13 13:42:58 on ttys000 env127:~ Genome$ I want to exit from the above to enter ...
software error written 11 months ago by dasakan0 • updated 11 months ago by zx87548.0k
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MIPgen probe design for SNP target resequencing-help
... Hi, I am intending to do targeted resequencing for SNP variant analysis in different population by using MIP based sequencing using illumina platform. I have on hand, SNP containing fasta transcripts (generated previously), known SNP location and RefGenome i can download for Mouse as model. I am new ...
genome next-gen snp written 13 months ago by dasakan0

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