User: Francois

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Francois0
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Posts by Francois

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Comment: C: How to design primers with primer3_core
... Merci beaucoup Pierre ! ...
written 28 days ago by Francois0
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Comment: C: How to design primers with primer3_core
... Ok I got it. The "PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/my/path/to/primer3_config/" (with a trailing slash) should be written at the top of the sequence file. ...
written 28 days ago by Francois0
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Comment: C: How to design primers with primer3_core
... I took the same example as in the manual and I still have the same error.... ...
written 28 days ago by Francois0
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Comment: C: How to design primers with primer3_core
... sorry, it doesn't work....here is my whole command : $ PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/usr/local/bioinfo/src/Primer3/current/bin/primer3_config $ primer3_core < Sus_scrofa_IFNG_201_sequence.fa output: PRIMER_ERROR=thermodynamic approach chosen, but path to thermodynamic parameters not spe ...
written 28 days ago by Francois0
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Comment: C: How to design primers with primer3_core
... Thanks Pierre, do you mean : $ primer3_core < PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/my/path/to/primer3_config my_input_file ? The primer3_config directory is not found. However it's existing and containing the following files/directories : dangle.dh interpretations loops.ds stack.ds stac ...
written 28 days ago by Francois0
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How to design primers with primer3_core
... Hi, I want to use "primer3_core" to design PCR primers from a multi-fasta file. The --help says : $ primer3_core < my_input_file But output said : PRIMER_ERROR=thermodynamic approach chosen, but path to thermodynamic parameters not specified = Maybe it has something to do with the "PRIMER_THER ...
primers written 28 days ago by Francois0 • updated 28 days ago by Pierre Lindenbaum116k
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Comment: C: R package openxlsx and gradient
... data visualisation isn't related to bioinformatics? ...
written 5 weeks ago by Francois0
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R package openxlsx and gradient
... Hi, I use the R package "openxlsx" and the "conditionalFormatting" function. I can apply different colors to cells in the excel output file according to selected values. Now I'd like to apply a color gradient to a column in my dataframe instead of preselected color values. Is it possible with "co ...
R openxlsx written 5 weeks ago by Francois0 • updated 15 days ago by Biostar ♦♦ 20
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Comment: A: Heatmap : why scaling ?
... sorry to ask again my question, but could someone help me to understand data scaling? why couln't I find a standard protocol to draw a heatmap? ...
written 5 months ago by Francois0
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Comment: A: Heatmap : why scaling ?
... I understand better now what is the purpose of the "scaling". Nevertheless, I still don't understand its effect on my data values. For example the gene that has the highest FC value in one condition and so appears in red in a heatmap without scaling ('redgreen' heatmap color range), now appears in ...
written 5 months ago by Francois0

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