User: rnaseqbias

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rnaseqbias0
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Posts by rnaseqbias

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Sequence specific bias for reverse transcriptase
... Are there any known sequence-specific bias for reverse transcriptase? I am interested in the bias that may arise in the first strand cDNA synthesis step of RNA-Seq library preparation. Thank you. ...
rna-seq sequencing written 10 weeks ago by rnaseqbias0
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Comment: C: Cause of 3' bias in polyA selection step in RNASeq library prep
... Is the bias because of biochemical or mechanical reasons? If it is a purely biochemical process, then the 3' bias should also show up if ribo-depletion is used instead of polyA selection to get rid of ribosomal RNA.(But as far as I know this is not the case.) So is there a mechanical aspect to this ...
written 11 weeks ago by rnaseqbias0
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Cause of 3' bias in polyA selection step in RNASeq library prep
... Hello. What is the cause of 3' bias in the polyA selection step in RNASeq library preparation? (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310221/figure/F6/) I have searched around on the internet but could not find the mechanism and cause explicitly explained. Is there a mathematical model for t ...
rna-seq sequencing written 11 weeks ago by rnaseqbias0 • updated 11 weeks ago by h.mon20k

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