User: brett.vanderwerff

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b****************@wsu.edu

Posts by brett.vanderwerff

<prev • 5 results • page 1 of 1 • next >
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Answer: A: A question about how the kegga function or KEGG analysis in general determines s
... I am still interested in understanding how exactly these statistics are calculated, but I can tell now from just playing around with the function that if 5 genes out of 10 total sig DE genes are in my favorite pathway that will be more sig than if 5 genes out of 100 total sig DE geens are in my favo ...
written 5 weeks ago by brett.vanderwerff10
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A question about how the kegga function or KEGG analysis in general determines significance.
... Hello, Lets say I did an rna-seq experiment experiment with control and drug, I am interested in KEGG pathways that sig. respond to drug. I do KEGG analysis with the `kegga` function (https://tinyurl.com/y7eszpdo) by passing a list of genes sig. affected by drug as the `de` argument, indicating ...
kegga rna-seq kegg edger limma written 5 weeks ago by brett.vanderwerff10
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Comment: C: How to design qPCR primers to validate RNA-seq experiment?
... Thank you for responding. I have used Primer3, but my question is less about what primer tool to use and more about what transcript for a particular gene I should be designing my primers against. ...
written 4 months ago by brett.vanderwerff10
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Comment: C: How to design qPCR primers to validate RNA-seq experiment?
... Yes I am trying to validate analysis results, I was not sure how much detail was needed, In apologize for that I have updated my questions to better describe my pipeline. ...
written 4 months ago by brett.vanderwerff10
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How to design qPCR primers to validate RNA-seq experiment?
... Hello, I did a bulk mRNA-seq experiment where I treated human cells in vitro with control of drug. I am only interested in determining differential gene expression at the gene level between these groups. I isolated RNA from treated cells and used poly-A selection and did sequencing on the illumina ...
rna-seq qpcr written 4 months ago by brett.vanderwerff10 • updated 4 months ago by Malcolm.Cook940

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