User: brett.vanderwerff

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b****************@wsu.edu

Posts by brett.vanderwerff

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Comment: C: How to design qPCR primers to validate RNA-seq experiment?
... Thank you for responding. I have used Primer3, but my question is less about what primer tool to use and more about what transcript for a particular gene I should be designing my primers against. ...
written 11 days ago by brett.vanderwerff10
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Comment: C: How to design qPCR primers to validate RNA-seq experiment?
... Yes I am trying to validate analysis results, I was not sure how much detail was needed, In apologize for that I have updated my questions to better describe my pipeline. ...
written 11 days ago by brett.vanderwerff10
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How to design qPCR primers to validate RNA-seq experiment?
... Hello, I did a bulk mRNA-seq experiment where I treated human cells in vitro with control of drug. I am only interested in determining differential gene expression at the gene level between these groups. I isolated RNA from treated cells and used poly-A selection and did sequencing on the illumina ...
rna-seq qpcr written 11 days ago by brett.vanderwerff10 • updated 2 days ago by Malcolm.Cook820

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