User: conor93
conor93 • 20
- Reputation:
- 20
- Status:
- New User
- Location:
- Hong Kong
- Last seen:
- 5 days, 23 hours ago
- Joined:
- 5 months, 1 week ago
- Email:
- c******@hku.hk
Irish PhD Student in Hong Kong
Posts by conor93
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... Thanks Kevin, will do. ...
written 4 months ago by
conor93 • 20
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... Hi All,
I am about to undertake RNA-Seq analysis to assess the lncRNA expression induced in viral infected human cells.
Experimental Conditions:
I have three conditions that I need to test; wildtype virus infected, mutant virus infected, and uninfected.
I am able to analyse data up to and includ ...
written 4 months ago by
conor93 • 20
• updated
4 months ago by
Kevin Blighe ♦ 37k
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... Hi Kevin,
I can definitely identify this problem in regards to multi-mapped reads, but what i'd like to know is, is there any way to differentiate expression of lncRNA genes located at intronic sites of protein-coding genes, which are also simultaneously being expressed. I haven't been able to fi ...
written 5 months ago by
conor93 • 20
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... Hi Kevin,
I have used the filtering criteria as per your suggestions, but what i have recently noticed is that with some highly expressed lncRNA genes, particularly with intronic ones, they tend to overlap with highly expressed protein coding genes. When i look at the raw counts generated using lnc ...
written 5 months ago by
conor93 • 20
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... Hi Charles,
Thanks for getting back to me,
> Ideally, having a merged list prior to quantification is theoretically best, but you'd have to have some system for naming the overlapping annotations.
May I ask what system you mean when dealing with overlapping annotations?
Thanks,
Conor ...
written 5 months ago by
conor93 • 20
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... Hi All,
I am quite new to RNA-Seq analysis and I have some confusion as to how to perform certain filtering steps in my pipeline.
Experiment Conditions:
I have three conditions tested based on viral infection experiments in human cells; Uninfected, Wildtype Virus Infected, and Mutant Virus Infect ...
written 5 months ago by
conor93 • 20
• updated
5 months ago by
Charles Warden ♦ 6.1k
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