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User: conor93

gravatar for conor93
conor93 • 40
Reputation:
40
Status:
New User
Location:
Hong Kong
Last seen:
2 days, 15 hours ago
Joined:
11 months, 1 week ago
Email:
c******@hku.hk

Irish PhD Student in Hong Kong

Posts by conor93

<prev • 6 results • page 1 of 1 • next >
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Comment: C: LncRNA Differential Expression and Analysis of lncRNA and mRNA co-expression.
... Thanks Kevin, will do. ...
written 10 months ago by conor93 • 40
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LncRNA Differential Expression and Analysis of lncRNA and mRNA co-expression.
... Hi All, I am about to undertake RNA-Seq analysis to assess the lncRNA expression induced in viral infected human cells. Experimental Conditions: I have three conditions that I need to test; wildtype virus infected, mutant virus infected, and uninfected. I am able to analyse data up to and includ ...
co-expression alignment deseq2 rna-seq lncrna written 10 months ago by conor93 • 40 • updated 10 months ago by Kevin Blighe ♦ 46k
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Comment: C: Filtering in RNA-Seq to get Differentially Expressed lncRNA
... Hi Kevin, I can definitely identify this problem in regards to multi-mapped reads, but what i'd like to know is, is there any way to differentiate expression of lncRNA genes located at intronic sites of protein-coding genes, which are also simultaneously being expressed. I haven't been able to fi ...
written 11 months ago by conor93 • 40
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Comment: C: Filtering in RNA-Seq to get Differentially Expressed lncRNA
... Hi Kevin, I have used the filtering criteria as per your suggestions, but what i have recently noticed is that with some highly expressed lncRNA genes, particularly with intronic ones, they tend to overlap with highly expressed protein coding genes. When i look at the raw counts generated using lnc ...
written 11 months ago by conor93 • 40
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Comment: C: Filtering in RNA-Seq to get Differentially Expressed lncRNA
... Hi Charles, Thanks for getting back to me, > Ideally, having a merged list prior to quantification is theoretically best, but you'd have to have some system for naming the overlapping annotations. May I ask what system you mean when dealing with overlapping annotations? Thanks, Conor ...
written 11 months ago by conor93 • 40
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Filtering in RNA-Seq to get Differentially Expressed lncRNA
... Hi All, I am quite new to RNA-Seq analysis and I have some confusion as to how to perform certain filtering steps in my pipeline. Experiment Conditions: I have three conditions tested based on viral infection experiments in human cells; Uninfected, Wildtype Virus Infected, and Mutant Virus Infect ...
R rna-seq assembly written 11 months ago by conor93 • 40 • updated 11 months ago by Charles Warden ♦ 7.2k

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