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Posts by rasmus.agren
... I have an experiment with significant technical issues. There are large differences in RIN values, as well as in QC metrics from the sequencing. Coverage is bad and varying, with samples ranging from 2 - 6 M uniquely mapping reads (human samples). Unfortunately the samples are few and expensive, and ...
... I have a single-cell RNA-seq experiment with five different treatments. The treatments are likely to result in different cell types, although this isn't known at this stage of the analysis. Regardless, they can be assumed to be quite different. Due to technical problems the size factors are also ver ...
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