User: harrypotterandsbt

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Posts by harrypotterandsbt

<prev • 14 results • page 2 of 2 • next >
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Comment: C: ssRNA-seq:Why saying ignoring the orientation of transcription leads to an overe
... OK, What I want to express is that in stranded RNA-seq library preparation, the left cDNA first strands are still need to PCR before sequencing. So we still will synthesize the second strands ,but only the first strands are selected to sequencing. There must be a selective mechanism. And I think i ...
written 3 months ago by harrypotterandsbt10
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Comment: C: ssRNA-seq:Why saying ignoring the orientation of transcription leads to an overe
... Thank you Kevin Blighe! You have solved my first question.And Do you know the second question's answer? ...
written 3 months ago by harrypotterandsbt10
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ssRNA-seq:Why saying ignoring the orientation of transcription leads to an overestimation of expression level for genes where the level of reverse transcription is comparable with that of forward
... Hello everyone ,It 's my first question in biostars. Recently I was annoyed by a question about ssRNA-seq library preparation. there are two question : 1. we know that standard RNA-seq library preparation produce double-strand cDNA.Does two strands of cDNA all be sequenced in sequencing process ? I ...
strand-specific rna-seq library preparation written 3 months ago by harrypotterandsbt10 • updated 3 months ago by Kevin Blighe42k
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Comment: C: Trinotate Sqlite db error
... I meet same problem.Have you fixed it yet? ...
written 7 months ago by harrypotterandsbt10

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