User: haozhaozhe

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haozhaozhe0
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Posts by haozhaozhe

<prev • 6 results • page 1 of 1 • next >
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Comment: C: Sorting fq data into groups with missing barcode info?
... Thank you, swbarnes2! So I was only given this pile of data without knowing what the sequencing service did... Assume what you said is true (non-standard library preps that might put the sample barcode in read 2 instead of the usual index position), how should I proceed? ...
written 15 hours ago by haozhaozhe0
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Comment: C: Sorting fq data into groups with missing barcode info?
... Thank you, genomax! I agree centroid info seems not important. Put it here just in case... Nice to confirm. Your link is very useful. Apparently, it means I cannot simply separate files based on their header... I will try the package in the link! ...
written 1 day ago by haozhaozhe0
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Comment: C: Sorting fq data into groups with missing barcode info?
... What do you me by non-standard? This is my first time saw this kind of files, so not much to compare with.... ...
written 1 day ago by haozhaozhe0
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Comment: C: Sorting fq data into groups with missing barcode info?
... These are regular Illumina barcodes. We are running this by ourselves because we cannot reach the provider any more... So I am basically on my own. When you said that the barcode and reads do not match, do you mean their header line does not match? This is my bad. I did not paste the matching one ...
written 1 day ago by haozhaozhe0
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Comment: A: Sorting fq data into groups with missing barcode info?
... I am very sad to see that there are 89 views and no answer yet... Can someone maybe point to me how I can improve my post to make it clearer to the readers? Or what additional info I need to put in? Thanks! ...
written 1 day ago by haozhaozhe0
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Sorting fq data into groups with missing barcode info?
... Dear all, I am trying to split a pooled bacteria Illumina sequencing data by samples. i.e., we have 20 samples. The results from the 20 samples are grouped in one fq file. We are tring to spit it into 20 fq files, one for each sample. We also have a separate barcode file. And here are example ...
next-gen sequencing written 5 days ago by haozhaozhe0

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