User: eozcan
eozcan • 10
- Reputation:
- 10
- Status:
- New User
- Location:
- Last seen:
- 2 months, 3 weeks ago
- Joined:
- 2 years, 2 months ago
- Email:
- e*****@foodsci.umass.edu
Posts by eozcan
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1
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... Hi,
I know this question has been posted before but I could not find answers to increase my counts for RNA-seq reads. I have done RNA-seq in Illumina Nextseq. I prepared libraries with NEBNext Ultra II Directional library kit. I used bowtie2 to map my reads to the reference genome. It is a bacteri ...
written 2.1 years ago by
eozcan • 10
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... >NS500540:135:HFF77AFXY:1:11101:6499:1061 1:N:0:ATTACTCG+NGGATAGG
GGCATTACTTCGGGGTAGGTCTTAAATAAGAGCGCAATGTTGGTATAGGCTCCCGTTGGCACTAACGTAATCGGT
>NS500540:135:HFF77AFXY:1:11101:23445:1062 1:N:0:ATTACTCG+NGGATAGG
CGCGTTTAAAACTTGGGAATGTCTTCGTAACAGACATGCACCTCCGTTTAAACTTTGTAATACCTGAGT ...
written 2.1 years ago by
eozcan • 10
• updated
2.1 years ago by
WouterDeCoster ♦ 45k
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... I tried to upload a fasta concatenated file from my Illumina pair-end reads, it says "something went wrong displaying the webpage." ...
written 2.1 years ago by
eozcan • 10
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... here is the link. I tried fastq format, it reject that format from the beginning.
https://www.ezbiocloud.net/tools/contest16s ...
written 2.1 years ago by
eozcan • 10
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1.9k
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... Hi,
Anybody used ContEst16S: an algorithm that identifies contaminated prokaryotic genomes using 16S RNA gene sequences from EZBiocloud for Illumina files? It gives error when I upload fasta file reads from Illumina. I unzip files and convert them to fasta. It says something went wrong in a webpag ...
7
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... Hello,
I am trying to remove adapters from my RNA-seq files obtained from Illumina. I used NEBNext ultra directional kit for illumina for library prep and NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) for adaptors and primers. I want to use trimmomatic, it has adapter files fo ...
written 2.2 years ago by
eozcan • 10
• updated
11 months ago by
alexgoro13 • 0
Latest awards to eozcan
Popular Question
12 months ago,
created a question with more than 1,000 views.
For EzBioCloud for contamination of Illumina raw files
Popular Question
23 months ago,
created a question with more than 1,000 views.
For has anyone use trimmomatic with NEBNext adaptors
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