User: cats_dogs

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cats_dogs0
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Posts by cats_dogs

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Comment: C: estimating read length and SD for Kallisto (single-end QuantSeq reads)
... Ah, rats, okay. Thank you, you saved me from a fairly large goof. Do you think it's advisable to adjust the insert size for libraries with different fragment lengths in addition to doing a run with 100 bp for all? Cheers! ...
written 7 months ago by cats_dogs0
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Comment: C: estimating read length and SD for Kallisto (single-end QuantSeq reads)
... ah, thanks! noted for the future! no one had replied yet though ...
written 7 months ago by cats_dogs0
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estimating read length and SD for Kallisto (single-end QuantSeq reads)
... Hi all, Deleted and reposted this to ask an actually sensible question. Sorry to those who've had to see an iteration of this twice. I am intending to use Kallisto and was looking for an opinion re: estimating fragment length. We used a Lexogen 3’ QuantSeq kit. Here is a sample Bioanalyzer trace o ...
quantseq alignment kallisto lexogen rna-seq written 7 months ago by cats_dogs0 • updated 7 months ago by michael.ante3.3k

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