User: kousi31
kousi31 • 0
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Posts by kousi31
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... Pre-computed alignments are not available for domestic water buffalo (Bubalus bubalis) in UCSC or ensemble. I want to create a maf file for buffalo with human, cow and dog. Is there any tutorial links on how to perform multiple alignment on genomes? What may be memory requirement to do the same? ...
written 4 weeks ago by
kousi31 • 0
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... Hi all,
I have four replicates for early and late lactation stages each. I did rlog transformation of the htseq counts and did PCA as a QC step to know whether the biological conditions cluster together. I obtained the attached PCA plot. Now I have a confusion on the axis I have to look for. As in ...
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... Thank you for your suggestions. ...
written 4 weeks ago by
kousi31 • 0
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...
I tried `cpan App::cpanminus`
then `sudo cpanm Genecare::PerlOligos`
Got error
> Finding Genecare::PerlOligos on cpanmetadb failed. ! Finding
> Genecare::PerlOligos () on mirror http://www.cpan.org failed. !
> Couldn't find module or a distribution Genecare::PerlOligos
The link sa ...
written 4 weeks ago by
kousi31 • 0
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5 follow
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... Hi all,
I want to install LncTar software.
I have downloaded LncTar as compressed file.
I extracted the folder. The folder has
LncTar.pl file and Genecare folder
Inside the Genecare folder there is a file named - PerlOligos.pm
when I run the command
`perl LncTar.pl -p 1 - l Examp1_RNA1. ...
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... Can you please tell me what would be the RAM requirement, If I attempt to do a pairwise alignment between human and buffalo genome (2.7 GB) using LASTZ?.. ...
written 5 weeks ago by
kousi31 • 0
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... Hi all,
I am trying to install FIRE.
I have installed pcre-8.42 in the directory `/usr/local/src.`
But upon running `./configure` I get the error
> "Attention: you don't appear to have libpcre installed on your
> machine."
I ran `sudo sh install-pcre.sh`(provided by the software itself)
...
written 6 weeks ago by
kousi31 • 0
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... A clear tutorial. I need water buffalo (Bubalus bubalis) and it is not in ensemble too. ...
written 6 weeks ago by
kousi31 • 0
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... Hi all,
I want multiple sequence alignments of few vertebrate species (Human, cow, buffalo, sheep and goats). UCSC browser has MSA for 46 vertebrate species and MSA of 3 or 4 species under individual species. But I don't find the combination I need.
Is it possible to extrat MSA of desired species ...
written 6 weeks ago by
kousi31 • 0
• updated
6 weeks ago by
andrew.j.skelton73 ♦ 5.8k
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... Thank you for your patient suggestions. ...
written 7 weeks ago by
kousi31 • 0
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8 weeks ago,
created an answer that has been accepted.
For C: stringtie2 error : ./samtools-0.1.18/bgzf.h:29:10: fatal error: zlib.h: No such
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