User: pablo_iturralde

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Posts by pablo_iturralde

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Removing artifact from evolved tuxedo pipeline
... Greetings, I've processed a data set that had ribosomal RNA contamination through the evolved tuxedo pipeline (see picture below) after removing rRNA using bbduk from bbmap: [Pipeline for Ribbosomal RNA contaminated reads][1] Once I started visualizing volcano plots I noticed some strange artifac ...
rna-seq written 13 months ago by pablo_iturralde0
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Comment: C: Log2 FC vs log2 FC scatterplot comparison between RNA-Seq data replicates
... Thanks for the response, that is what I've been advised also! So, the take home message is that when observing fold change it could be noisy, therefore not the best correlational strategy? ...
written 17 months ago by pablo_iturralde0
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Comment: C: Log2 FC vs log2 FC scatterplot comparison between RNA-Seq data replicates
... I'm comparing fold-change of treated rep1 vs control with fold_change of treated rep2 vs control ...
written 17 months ago by pablo_iturralde0
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Log2 FC vs log2 FC scatterplot comparison between RNA-Seq data replicates
... Hello, I need to clarify a theoretical/conceptual analytical approach. Based on my understanding of the fold change patterns observed in an RNA-seq DE experiment. When successful, one gathers very unique patterns of gene/transcript expression which are observed when plotted on a scatterplot. When ...
rna-seq written 17 months ago by pablo_iturralde0 • updated 16 months ago by Biostar ♦♦ 20

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