User: sabaghianamir70

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Posts by sabaghianamir70

<prev • 23 results • page 1 of 3 • next >
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how can i download human ribosomal reference ?
... is there any such data ? and if it is, how can i download it . i need it for extract ribosomal rna from RNA seq data . ...
rrna rna-seq written 18 hours ago by sabaghianamir700 • updated 16 hours ago by genomax67k
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Comment: C: Find illumina adaptors
... thats what i dont know, how should i found out which kit used on my data ? i just saw the NEXTseq500 ...
written 18 hours ago by sabaghianamir700
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Find illumina adaptors
... i have a data produce from nextseq 500 and i want to find the adapters which used in my data, how do i find that adapter in https://support.illumina.com/downloads/illumina-adapter-sequences-document-1000000002694.html ...
rnaseq ilumina trimming cutadapt written 21 hours ago by sabaghianamir700 • updated 16 hours ago by genomax67k
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Comment: C: Identify and separate Micronas from my raw data.
... its Rna SEQ single end illumina data generated with nextseq 500. cell line is blood. after Triming i used Star and feature count. but i donw know it is a correct work flow or the options of these tool are correct also or not. ...
written 7 days ago by sabaghianamir700
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Identify and separate Micronas from my raw data.
... i want to identify and separate miRNAs from my Raw data. how should i do that? in fact after i analyze the data what tools and options should i use to identify and separate microRNA from my data. for example after QC the Data, should i use Star with some specific options for this matter or should i ...
rna-seq microrna mirna written 8 days ago by sabaghianamir700
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Comment: C: What is the best tool for estimate the counts of genes with very low expression
... if i want to get maximum amount of reads, which means map every read even the false one, So. first.:what option should i use(salmon options)? and second: i want filter the false reads with FDR and p value .(is it Right strategy?).i asking because i think you are working on this matter. ...
written 8 days ago by sabaghianamir700
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Comment: C: What is the best tool for estimate the counts of genes with very low expression
... i edited my post. i think it is more clear now ...
written 10 days ago by sabaghianamir700
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What is the best tool for estimate the counts of genes with very low expression rate ?
... i looking for a tool which can give me "count number" for very low expression rate genes or pseudogenes like PER3. What is the best tool for estimate the counts of genes with very low expression rate ? or the best aligner or aligner options to get me maximum amount of fragments to recognize with a F ...
gene tools alignment rna-seq written 10 days ago by sabaghianamir700
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Comment: C: How should i determine outFilterMultimapNmax in rna seq analysis, P.s im working
... im working on rna sequencing project, and i want to find transcripts of pseudogenes with very low expression rate, but im tried that before and i get zero counts,(using Star, FeatureCount not htseq and limma for the DEG), i want to know what options i must set, to get some count from it.(some one to ...
written 29 days ago by sabaghianamir700
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How should i determine outFilterMultimapNmax in rna seq analysis, P.s im working on pseudogenes.
... how do i know what the outFilterMultimapNmax must be for my analysis? consider that,i am working on pseudogenes. ...
pseudogenes star rna-seq written 4 weeks ago by sabaghianamir700 • updated 4 weeks ago by h.mon25k

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