User: sabaghianamir70
sabaghianamir70 • 10
- Reputation:
- 10
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- New User
- Location:
- iran
- Last seen:
- 2 days ago
- Joined:
- 8 months, 4 weeks ago
- Email:
- s**************@gmail.com
Phd student in molecular medicine
Posts by sabaghianamir70
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... Hi,
I Did a differential expression with the result of `MiRDeep2` Mapper process and map reads to a reference genome in galaxy, with salmon and then use `Deseq2` for DEG. is this workflow OK ? or im making mistake in calculation. if there is better way can you guys tell me about it. P.s i didnt get ...
written 3 days ago by
sabaghianamir70 • 10
• updated
3 days ago by
ATpoint ♦ 22k
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... Hi everyone
Does anyone know about a valid facility for Courses to learning more RNA sequencing analysis as winter school or some thing like that ? I know this is not a scientific question but i really need some answers from the experts.
Thanks ...
written 7 days ago by
sabaghianamir70 • 10
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... i know ,your right, but take a look to this SRR file . then you see whats im talking about... SRR3474262 ...
written 5 weeks ago by
sabaghianamir70 • 10
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... Hey Guys
I want to get count from a circadian gene which is PER4. it is PER3s pseudogene.
So i used Salmon and ive got Per3 not per4 Counts. But i saw some data in same paper which the name was Chromosome conformation capture(Hi-C) which has Counts in per4. So is this right to do DEG on Hi-C inst ...
written 5 weeks ago by
sabaghianamir70 • 10
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... Hi every one
I doing a friend a favor about his project,
He has only 2 dataset, one of them is control and one is treat, those are pair end data and he does not had the budget to send more sample for rna sequencing. So anyway i did align those data and finally i cant find any tool for differentia ...
written 7 weeks ago by
sabaghianamir70 • 10
• updated
7 weeks ago by
RamRS ♦ 24k
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... since i working in galaxy plat form . working with galaxy is a little confusing and different from other tools i used. because it using data without needing to aligner. what the exact steps of Salmon ? i mean after trimming. we use salmon and featureCounts? i dont know what workflow should i use ...
written 3 months ago by
sabaghianamir70 • 10
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... i know these two are similar. but im make my point more clear this time. thank you anyway ...
written 3 months ago by
sabaghianamir70 • 10
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... Hi every one.
i did rna seq analysis. in PER3 gene and PER4 pseudogene i didnt get any counts, but in IGV i saw some small peaks which means they have some counts, even very low, but still they have !!. i want to know is there any way i can get any counts from that ? were my options in my aligner w ...
written 3 months ago by
sabaghianamir70 • 10
• updated
3 months ago by
swbarnes2 ♦ 6.5k
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... thank you my friend <3 ...
written 3 months ago by
sabaghianamir70 • 10
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... hi every one
i was looking for a rnaseq data, which has a time seris for example 0-24 hour or longer. i searched my self a find a few. but i asking to if anyone know any paper which can help me. ...
written 3 months ago by
sabaghianamir70 • 10
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