User: joannerandy80

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Posts by joannerandy80

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Comment: C: How can I get the sequence of 5' and 3' UTR regions of bacterial genome?
... Thanks a lot.. let me give a try.. ...
written 4 weeks ago by joannerandy8010
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Comment: C: How can I get the sequence of 5' and 3' UTR regions of bacterial genome?
... Thanks.. I believe you mean by "range from the beginning of the gene..." actually "range from the beginning of the transcript...". But my problem begins how to define / identify / the beginning of the transcript and end of the transcript.. As explained below, my aim is to find accessible open areas ...
written 5 weeks ago by joannerandy8010
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Comment: C: How can I get the sequence of 5' and 3' UTR regions of bacterial genome?
... Thanks Healey. I am trying to find accessible open areas in the mRNA secondary structures of a few bacterial genes (Ex. Alr, dxr) to identify Antisense oligonucleotides (ASOs) binding target. There are few programmes available to predict the secondary structure but we need to key in exactly entire m ...
written 5 weeks ago by joannerandy8010
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How can I get the sequence of 5' and 3' UTR regions of bacterial genome?
... Hi there, Is there any software tool or database to identify the entire 5' and 3' UTR regions of bacterial gene? I am aware that eukaryotic genes are clearly annotated in Ensembl and Genbank with these details. But unfortunately I couldnt able to find this information for bacterial genes. Your help ...
genome gene sequence rna-seq assembly written 5 weeks ago by joannerandy8010
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Shine Dalgarno Sequence identification
... Hi there, could any of you please enlighten me how can I find the exact shine-dalgarno sequence of a specific bacterial gene. It seems like many bacterial genes do not have the classic AGGAGGU consensus sequence in their 5'UTR region. Is there any database or software tool available to find this reg ...
genome rna-seq written 5 weeks ago by joannerandy8010

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