User: marc.osullivan

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Posts by marc.osullivan

<prev • 5 results • page 1 of 1 • next >
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Suggested analysis on single patient with methylation data and multiple timepoints
... I have a single patient with multiple time points and high-throughput data corresponding to each timepoint, and I'm wondering what the best R pipeline or statistical method would be to find the markers that change the most across time? ...
R microarray written 11 weeks ago by marc.osullivan10
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Comment: C: RNA sequencing with metaboanalyst
... I would ensure you're using raw read counts, to my knowledge, it's not possible to have negative raw read counts. Ensure you have sample normalisation and autoscaling to set to none as both have been down with the VST and scale function in DeSeq2 ...
written 11 weeks ago by marc.osullivan10
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Comment: C: RNA sequencing with metaboanalyst
... Thanks for your help Kevin; your help is much appreciated I'm now quite confident with most of the steps thanks to your advice. ...
written 5 months ago by marc.osullivan10
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Comment: C: RNA sequencing with metaboanalyst
... Thanks for your response Kevin, Unfortunately, I'm new to "big data" statistics and R studio but I'm slowly getting the hang of R studio. I'd like to ask you two more questions based on your answer if possible: Can you recommend from your own experience what posthoc transformation would be best su ...
written 5 months ago by marc.osullivan10
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RNA sequencing with metaboanalyst
... I'm currently trying to input my RNA sequencing data into metaboanalyst.ca as in this article: https://jamanetwork.com/journals/jamapediatrics/fullarticle/2663880 Can anyone recommend if I should use QC raw counts or the normalised counts in my data file that I input? Can anyone recommend what nor ...
R rna-seq sequencing written 5 months ago by marc.osullivan10 • updated 5 months ago by Kevin Blighe45k

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