User: Dave Th
Dave Th • 10
- Reputation:
- 10
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- New User
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- Last seen:
- 4 weeks ago
- Joined:
- 9 months, 1 week ago
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Posts by Dave Th
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... Hi all,
I'm trying to filter my contigs dataset into different files by their length such as 500bp, 1kb, 2kb... I'm using below code to produce my output.
def contigs_filter_by_length(fasta_input, size, fasta_output):
long_contigs = [] #Create an empty list
for record in SeqIO.parse(f ...
written 29 days ago by
Dave Th • 10
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... Hi all,
I would like to use MEGAN for my metagenomics functional analysis. I have downloaded all of the mapping files from MEGAN website and meganized my DAA files. Taxonomy assignment works with all sequences, IP2G assignment also works with more than 75% sequences, but SEED and COG showed almost ...
written 8 months ago by
Dave Th • 10
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Comment:
C: MEGAN6 and Daa files from DIAMOND
... I already tried meganizer tool but it said Daa is not Daa file. Yesterday, I found out that the Daa file I got from DIAMOND is not actually DAA file because I used -o output.daa instead of -a output.daa. It's OK now but the number of sequences assigned to SEED is too low. It might because of my sequ ...
written 8 months ago by
Dave Th • 10
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... Hi all,
I am currently using MEGAN for my soil metagenomics. After using the latest version DIAMOND to get the DAA files, I imported these files to MEGAN along with GI mapping files (GI-Taxo, GI-KEGG, GI-SEED) downloaded from MEGAN6 website. The results show none of my sequences can be assigned to ...
written 8 months ago by
Dave Th • 10
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... Hi guys,
I want to use DIAMOND for my metagenome Functional analysis. As the instruction, I have to download NCBI nr database (ftp://ftp.ncbi.nlm.nih.gov/blast/db/). Unfortunately, my internet connection is not very stable, so I have to download a multiple nr file nr.**.tar.gz instead of a nr singl ...
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