User: mor.chalabi

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mor.chalabi10
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Posts by mor.chalabi

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How single cells are sorted in an scRNA-Seq protocol by FACS?
... I am computational guy trying to understand how FACS sorting works in an scRNA-Seq protocol. About sorting single cells in each well of a 384-well plate, I have a question that would be grateful if you could answer it. The protocol reads that cells are sorted in the wells based on their marker leve ...
scrna-seq facs flow cytomertry cell sorting written 4 weeks ago by mor.chalabi10
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Comment: C: What does the final RNA-Seq library for sequencing by Illumina contain? ss cDNA
... Thanks for your answer :-) you are right. I just read NextSeq 500 System Guide. The denaturation step is done by lab technician. ...
written 4 weeks ago by mor.chalabi10
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Comment: C: What does the final RNA-Seq library for sequencing by Illumina contain? ss cDNA
... Thank you for your answer. Then, why isn't included in the TruSeq protocol? Even when I read scRNA-seq protocol ( like Mars-Seq 2), the last step in library prep is again elongation. ...
written 4 weeks ago by mor.chalabi10
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What does the final RNA-Seq library for sequencing by Illumina contain? ss cDNA or ds cDNA?
... - I guess the oligos on Illumina's flow cell only hybridize to to single-stranded cDNAs (sscDNA). - However, when I read the TruSeq protocol (googled: TruSeq® RNASample Preparation v2 Guide) at the PCR step (AmpPCR, page 41) it looks like that the last cycle (d) is just elongation. - Therefore, si ...
illumina next-gen pcr rna-seq sequencing written 4 weeks ago by mor.chalabi10 • updated 4 weeks ago by ATpoint23k

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