User: saddamhusain77

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Posts by saddamhusain77

<prev • 8 results • page 1 of 1 • next >
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Comment: C: Aligned read loss after SAM to BAM conversion?
... @RamRS, not exactly as last name contains double 'S'. I hope it wouldn't have affected Biostars name policy if there is one, nor my ability to put or being answered to a question. ...
written 4 weeks ago by saddamhusain770
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Comment: C: Aligned read loss after SAM to BAM conversion?
... Thanks @swbarnes2, I get it. I am sorry but I don't get what you mean by "Posting that username on a public board is certainly a ...choice" ...
written 4 weeks ago by saddamhusain770
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Aligned read loss after SAM to BAM conversion?
... Hello there, I have aligned the sequenced reads with Bowtie aligner. After that I converted SAM output to BAM but when I checked the number of reads that were in BAM file I found a huge discrepancy in number of reads in SAM and BAM file. Is it normal to have reduced number of reads in BAM due to com ...
samtools alignment chip-seq written 4 weeks ago by saddamhusain770 • updated 4 weeks ago by swbarnes28.9k
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Comment: C: Discrepancy in differentially expressed genes p-adj
... Thank you for the responses, even if the multiple testing is the source of discrepancy, the analysis in the later case is underestimating the true positive results. In such a case what should be the appropriate approach to detect all the true positives? I mean, had I have done the analysis with GENC ...
written 18 months ago by saddamhusain770
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Comment: C: Discrepancy in differentially expressed genes p-adj
... Thank you for the response. ...
written 18 months ago by saddamhusain770
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Comment: C: Discrepancy in differentially expressed genes p-adj
... Thank you for the responses, even if the multiple testing is the source of discrepancy, the analysis in the later case is underestimating the true positive results. In such a case what should be the appropriate approach to detect all the true positives? I mean, had I have done the analysis with GENC ...
written 18 months ago by saddamhusain770
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Comment: C: Discrepancy in differentially expressed genes p-adj
... ~76000 in UCSC knowngene and 206694 in GENECODE if that is what you asked. ...
written 18 months ago by saddamhusain770
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Discrepancy in differentially expressed genes p-adj
... Hi, I analysed RNA seq data recently using UCSC knowngene as annotation file and found several differentially expressed genes. Then I confirm DEGs (P-adj<= 0.05) by real-time PCR and found consistency between rna-seq data and real-time PCR data. But when I analysed same data using same analysis ...
p-adj cutoff rna-seq written 18 months ago by saddamhusain770

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