User: scheme4193

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scheme419340
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Posts by scheme4193

<prev • 28 results • page 1 of 3 • next >
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Comment: C: Which Normalization is good in RNA-Seq ? How normalization is calculated ?
... What is the main difference between "scaling" and "normalization" ? ...
written 8 days ago by scheme419340
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Comment: C: Which Normalization is good in RNA-Seq ? How normalization is calculated ?
... Thanks. So, in bulk RNA-Seq, processing raw count matrix by vst or rlog before any analisys is standard, right ? ...
written 8 days ago by scheme419340
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Which Normalization is good in RNA-Seq ? How normalization is calculated ?
... Hi, everyone. I want to ask "normalization". This is very confusing term for me. I suppose basic bulk RNA-Seq pipeline, like hisat2 → featureCounts → DESeq2. In this situation, I want to draw PCA, dendrogram, co-scatter plot and heatmap. Now, I am using normalization like below. - PCA analysis:R ...
R rna-seq written 8 days ago by scheme419340
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Comment: C: How to adjust graphics co-scatter plot using R function "pairs.panels" in librar
... Thank you Kevin. It was a great help and I could solve my problem ! ...
written 16 days ago by scheme419340
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How to adjust graphics co-scatter plot using R function "pairs.panels" in library "psych" ?
... Hi, everyone ! I am drawing co-scatter plot with correlation coefficient using R function "pairs.panels" in library "psych". It is like this https://camo.qiitausercontent.com/8c9673bfde6691edde4465c6ff43c7c893709c9b/68747470733a2f2f71696974612d696d6167652d73746f72652e73332e616d617a6f6e6177732e636 ...
R graph rna-seq written 17 days ago by scheme419340 • updated 17 days ago by Kevin Blighe48k
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Comment: C: Error of featureCounts : /lib64/libc.so.6: version `GLIBC_2.14' not found (requ
... Thank you for your answering. I compiled on my enviroment, but computation should be run on different environment, i.e. I used supercomputer as university. But, I will try compile one more time. ...
written 6 weeks ago by scheme419340
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Comment: C: Error of featureCounts : /lib64/libc.so.6: version `GLIBC_2.14' not found (requ
... Thank you both. I feel sorry for not specifying my environment. Maybe, my environment is the center of this problem. Now, I am using supercomputer, So, I wrote command with shell script in .sh file and ran by using "qsub" command. So, though my environment has latest version of GLIBC, it may not be ...
written 6 weeks ago by scheme419340
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Error of featureCounts : /lib64/libc.so.6: version `GLIBC_2.14' not found (required by featureCounts)
... Hey everyone. When I ran featureCounts from sam file made by hisat2, I got error like below. > featureCounts: /lib64/libc.so.6: version `GLIBC_2.14' not found (required by featureCounts) I searched this error, and I thought my GLIBC version may be old. So, I checked by "ldd --version" >ld ...
software error next-gen rna-seq written 6 weeks ago by scheme419340 • updated 6 weeks ago by 2nelly170
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Comment: C: How to proccess Microarrays CEL file byR package "oligo" ?
... Thank you so much ! I will read it. ...
written 6 weeks ago by scheme419340
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How to proccess Microarrays CEL file byR package "oligo" ?
... Hello, everyone. I tried micro array analysis by R library "affy", but I got error like this. > eset <- mas5(hoge) > background correction: mas > PM/MM correction : mas > expression values: mas > background correcting...Error in getCdfInfo(object) : Could not obtain CDF env ...
software error R microarray written 6 weeks ago by scheme419340 • updated 6 weeks ago by Kevin Blighe48k

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