User: sarfrajiiet

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Posts by sarfrajiiet

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Comment: C: htseq-count: error: the following arguments are required: featuresfilename
... i am using HG38 for alignment ...
written 6 weeks ago by sarfrajiiet0
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Comment: C: htseq-count: error: the following arguments are required: featuresfilename
... ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_30/gencode.v30.long_noncoding_RNAs.gff3.gz I AM USING THIS IS CORRECT ...
written 7 weeks ago by sarfrajiiet0
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Comment: C: htseq-count: error: the following arguments are required: featuresfilename
... Thank you for your reply I have human rna sequence( paired ) so from where I get this gff file for this and then what the command I have to follow ...
written 7 weeks ago by sarfrajiiet0
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htseq-count: error: the following arguments are required: featuresfilename
... htseq-count -f bam -r name -i gene_id -s no -t exon /Users/abc/sorted.bam > count.txt > usage: htseq-count [options] alignment_file gff_file > htseq-count: error: the following arguments are required: featuresfilename not getting what's wrong in my code help me ...
alignment sequence rna-seq sequencing written 7 weeks ago by sarfrajiiet0 • updated 10 days ago by h.mon26k
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rna paired sequence sort is important
... i have rna paired fasta file I trimmed it using trim galore and then mapped it with hg38 bowtie2 index now i have sam file is it important to sort it for htseq count i have to ask that the process that i follow till now is it correct differential expression and second that what next to do to get d ...
sequence alignment rna-seq written 7 weeks ago by sarfrajiiet0 • updated 10 days ago by WouterDeCoster39k

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