User: Floyd

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Floyd0
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Posts by Floyd

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Comment: C: High G and low A,C,T content in the 1-10bp of Read2 file in a paired-end whole g
... Thank you so much for your answer! I will use those programs to have a try. I will also test omitting the first 15 bases. ...
written 14 months ago by Floyd0
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High G and low A,C,T content in the 1-10bp of Read2 file in a paired-end whole genome bisulfite sequencing, why?
... Hi there! I'm new to bisulfite sequencing. Recently received my paired-end WGBS raw file. The Read1 looks OK after fastQC, but the Read2 file has a waired 'Per base sequence content' figure. Why is there a such G content. It is supposed to be very low G reading due to C-T conversion in Read1 file. ...
bisulfite sequencing paired-end wgbs sequencing written 14 months ago by Floyd0 • updated 14 months ago by Devon Ryan97k

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