User: mxlsherry1992

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Posts by mxlsherry1992

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Answer: A: grep extract command will remove replicates automatically
... Solved!! and Vlookup in excel could also do this. Thanks!! ...
written 6 weeks ago by mxlsherry199230
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grep extract command will remove replicates automatically
... Dear all, I have a `file 1`, containing many gene ID, and also has replicates for the gene ID. I want to extract the corresponding line from an `gff file`, I have a script can accomplish this, but this script will remove the replicates automatically, if I don't want to remove the replicates and wan ...
rna-seq written 6 weeks ago by mxlsherry199230
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Comment: C: sort command question
... Thank u!! it worked!! ...
written 6 weeks ago by mxlsherry199230
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Comment: C: sort command question
... Thank you! it worked ...
written 6 weeks ago by mxlsherry199230
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sort command question
... Dear all, I had a "txt" format file, there are 6 columns in this file, and each column is separated by 'tab'. What I want to do is that I need to sort according to the third column (weight) and make it in descending order. Here is the script I used: `sort -t ' ' -k 3nr file.txt > output.txt` Bu ...
rna-seq written 6 weeks ago by mxlsherry199230 • updated 6 weeks ago by Mensur Dlakic3.5k
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Comment: C: extracting the corresponding ID from two files in linux
... Thank you!! it worked for me ! ...
written 6 weeks ago by mxlsherry199230
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extracting the corresponding ID from two files in linux
... Dear all, Assuming that I have two txt files, File 1 has many rows, each row containing many ID which is separated using "/", like this: 30676/30711/30712/30725/58038/58118 58152/58153/64269/64272/64273/64609/64610 30148/30154/30592/30696/30724 .... And File 2, the Column 1 contai ...
rna-seq written 7 weeks ago by mxlsherry199230 • updated 7 weeks ago by Joe16k
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identifying the expressed gene number in each timepoint
... Dear all, we have the RNA-SEQ data for 7 timepoints for a species, and this species has a reference genome. I found some papers will identify the gene number in each timepoint, but they used different method. 1. Some "an ad hoc cutoff for detectable expression was set at >=2 reads per transcript ...
rna-seq written 9 weeks ago by mxlsherry199230 • updated 4 weeks ago by dsull910
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Comment: C: NR annotation and Uniprot annotation
... Thank you so much!! pretty clear, and if for the publication, if these are important sequences, what I should rely on is the uniprot database, right..? I can not use both annotations since it is different .... Thank you so much!! ...
written 11 weeks ago by mxlsherry199230
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NR annotation and Uniprot annotation
... Hi all, I am doing the de novo assembly analysis, and I want to annotate the sequences using NR database and Uniprot database, but after annotation, for the same sequence, I found that it had different annotation meaning in NR and Uniprot database. Like this: If we annotate `TRINITY_DN129938_c2_g1 ...
rna-seq written 11 weeks ago by mxlsherry199230 • updated 11 weeks ago by prince2612199170

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Rising Star 6 months ago, created 50 posts within first three months of joining.

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