User: piotr.majewski

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Posts by piotr.majewski

<prev • 6 results • page 1 of 1 • next >
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Comment: C: Unicycler - hybrid assembly failure
... I am expecting genome somewhere around 5 Mb. In case of input files, nanopore data is quite extensive 1) long reads - 2.3 Gb 2) short reads R1 - 0.17 Gb 3) short reads R2 - 0.16 Gb ...
written 12 months ago by piotr.majewski0
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Comment: C: Unicycler - hybrid assembly failure
... I've forgot to mention that I am running analyses on Galaxy server. 16GB RAM will be enough to run it offline? ...
written 12 months ago by piotr.majewski0
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Comment: C: Unicycler - hybrid assembly failure
... I am currently using 46.5 GB out of total 250.0 GB space. ...
written 12 months ago by piotr.majewski0
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Unicycler - hybrid assembly failure
... Dear All, I've recently encountered some issues with Unicycler assembly. I've tried to perform hybrid assembly with use of 1) trimmed Illumina reads (R1+R2); format: fastqsanger.gz 2) nanopore reads; format: fasqsanger Unicycler readily deals with individual assembly of either Illumina or Nanopo ...
genome assembly next-gen software error sequencing written 12 months ago by piotr.majewski0 • updated 12 months ago by Joe16k
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plasmid recovery from hybrid assembly
... Dear All, After hybrid Unicycler assembly, I've received 3 contigs 1 (4.35 Mb) - Alcaligenes faecalis genome, circular (via Geneious) 2 (43.5 kb) - plasmid?, circular (via Geneious) 3 (11 kb) - ? how can I be sure that contigs 2&3 stands for plasmids? or maybe single plasmid divided into 2 ...
genome assembly next-gen sequencing written 12 months ago by piotr.majewski0 • updated 12 months ago by predeus1.3k
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evaluation of sequencing coverage depth
... Dear All, At the outset, I would like to point out that I am an amateur in the field of NGS. I've performed sequencing of prokaryotic genome (Alcaligenes faecalis with use of short (Illumina) and long reads (Nanopore). My goal is to fully resolve genome and mobilome structures with use of hybrid a ...
genome assembly next-gen sequencing written 13 months ago by piotr.majewski0

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