User: dt

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dt30
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Posts by dt

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3
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Answer: A: Bbduk filters away good reads
... Ok, I believe I figured it out, it's explained in https://github.com/wdecoster/NanoPlot/issues/57: > BBDuk calculates average quality score by converting to probability > scale, taking an average, and then converting back to Phred scale. So > for example, a 2bp read with quality scores 10 ...
written 9 days ago by dt30
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Comment: C: Bbduk filters away good reads
... Not really, I provided the exact read and the exact command to reproduce the problem. Can you reproduce it? ...
written 9 days ago by dt30
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Bbduk filters away good reads
... Hi, I seem to be missing something obvious, but I have a read (actually lots of reads, it's just an example) that shouldn't be filtered by bbduk.sh based on average quality, but it is. Read: @NZ_AP014881.1_0_0/2 TGCAGCATTCTCCTGATGGCGGTCTTGATGAAGAGCTTTGTTACGGGGGTCATCCTCATCCATCAGGTCTGGTGCA ...
preprocessing next-gen quality control filtering written 9 days ago by dt30

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Teacher 9 days ago, created an answer with at least 3 up-votes. For A: Bbduk filters away good reads

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