User: j.thomas93

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j.thomas930
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Posts by j.thomas93

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Comment: C: Any help on how to detect viral transcripts in ONT RNAseq data
... I was wondering if you could help me set up the command? What parameters would you recommend for minimap2 for my data and why? ...
written 5 days ago by j.thomas930
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Comment: C: Any help on how to detect viral transcripts in ONT RNAseq data
... Thanks for the help! I do indeed know what virus to look for so I will try the simultaneous alignment as you suggested! Thanks again! ...
written 5 days ago by j.thomas930
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Any help on how to detect viral transcripts in ONT RNAseq data
... Hiya, Im quite new to bioinformatics and linux in general. I have generated some RNAseq data using ONT minion 1d cDNA kit (PCS-109) to compare the transcriptome of cells expressing viral proteins which are stably transfected into the cells. As a control i just need to confirm the expression of thes ...
rna-seq ont written 5 days ago by j.thomas930 • updated 5 days ago by WouterDeCoster39k
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How to randomly subset a specific number of sequences from a large fastq file?
... Hi, So I am doing some RNA seq and I have a number of large library preps for the different conditions and replicates. In some samples there a huge differences in library size and I was hoping to just do a preliminary analysis where I randomly subset x amount of reads from the parent fastq, but i w ...
tool rna-seq written 13 days ago by j.thomas930 • updated 13 days ago by benformatics870

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