User: emilybowie2

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Posts by emilybowie2

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Comment: C: see reads in .bam file, no counts after HTseq
... I appreciate the link for asking good questions - I'll elaborate the steps/commands: - - Since I am a newbie, I uploaded all .fastq files to Galaxy - changed the file type to .fastqsanger before using Trim Galore. I used Trim Galore via Galaxy. My sequences were single-end, I chose automatic adap ...
written 5 days ago by emilybowie20
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see reads in .bam file, no counts after HTseq
... Hi! VERY, VERY (so please be nice to me!) new to the field here, but briefly: performed RNAseq (mouse tissue), got the sequences (fastq format), cleaned them up (trimgalore), aligned them (RNASTAR to mm10) - checked alignments on IGV and can see sufficient reads across all exons for GOI, however wh ...
htseq counts rna-seq written 5 days ago by emilybowie20

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