User: sambunga094

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Posts by sambunga094

<prev • 10 results • page 1 of 1 • next >
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Answer: A: Differentially Expressed Genes Heatmap
... ![This is the plot i got. Top Differentially expressed genes for cluster 1. I want to plot against all the other clusters, but not the cells][1] https://ibb.co/7XKkQDc ...
written 4 months ago by sambunga0940
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Comment: C: Deferentially Expressed Genes Heatmap
... My bad, that's a typo. Sorry! ...
written 4 months ago by sambunga0940
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Differentially Expressed Genes Heatmap
... Hi, Can anybody help in how can i draw a heat map for DE genes for every Cluster for on expression data. I tried few packages in R but no luck. Help is highly appreciated! ...
R alignment rna-seq sequencing written 4 months ago by sambunga0940
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Comment: C: SC-RNA Seq Analysis tutorials
... Thank you Jared for you answer. I will look into it ...
written 5 months ago by sambunga0940
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SC-RNA Seq Analysis tutorials
... Hi, A very quick question.. Can anybody tell me where can i find tutorials for SC-RNA Seq analysis for QC, NOrmalization, Clustering and annotation apart from **SEURAT** . Thank you so much in advance!! ...
singlecell sc analysis seurat written 5 months ago by sambunga0940 • updated 5 months ago by jared.andrews075.3k
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Comment: C: How to append R1(Barcode+UMI) to header of R2(Read data)
... thanks for your reply. I want to process it without using cellranger. ...
written 8 months ago by sambunga0940
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How to append R1(Barcode+UMI) to header of R2(Read data)
... Hi, I am new to Bioinformatics, I have 10x Mouse 1k single cell brain dataset. How do i move the Barcode(16bp) + UMI(10bp) to header of R2 ? And also is this the right way to format a fastq file? PS: Im just learning how to process single-cell data. Please let me know if there are any tools avai ...
genome assembly alignment rna-seq sequencing written 8 months ago by sambunga0940
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Comment: C: How to align 10X R1 and R2 fastqs?
... I'm planning to build a matirx output so i can analyze it through seurat. Can i do cat of R1 and R2? Can you please explain me. Thank you so much! ...
written 8 months ago by sambunga0940
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Comment: C: How to align 10X R1 and R2 fastqs?
... This is mouse brain single cell data. https://support.10xgenomics.com/single-cell-gene-expression/datasets/3.0.0/neuron_1k_v2 ...
written 8 months ago by sambunga0940
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How to align 10X R1 and R2 fastqs?
... Hi guys, i am new to bioinformatics. Ill just keep my question simple. I have downloaded a fastq single cell dataset from 10X. It has R1 and R2 of lane 1 and lane 2. How can i align them and make it to one fastq file? Example: Input: neurons_mouse_L001_R1.fastq neurons_mouse_L002_R1.fastq ne ...
10x pairend fastq alignment rna-seq written 8 months ago by sambunga0940 • updated 8 months ago by Zhixue10

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