User: tiantai_wing

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Posts by tiantai_wing

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Comment: C: how to merge pair-end reads with maximum score 41 (J)
... Thanks all! bbmerge is so fast (merging in 2 min for 7 million paired-reads)! ...
written 6 weeks ago by tiantai_wing0
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how to merge pair-end reads with maximum score 41 (J)
... I want to merge my pair-end reads into one single reads with about 50 bp overlap. However, my fastq file is Illumina-1.8 Phred+33 format, so the maximum score is 41 (J). I tried NGmerge for merging but it can not expand to score 41 with -u 41 and editing the qual_profile. Is there any other method a ...
sequence assembly next-gen written 6 weeks ago by tiantai_wing0

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