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Posts by asim
... Really appreciate your suggestion, i will repeat PFGE, but should i further polish my assembled data. I mean may be assembled data having repeats. ...
... Hye, I sequenced whole genome of bacteria through pacbio 10kb. I assembled through CANU and got three contigs, two for plasmid and 1 for chromosome. I confirmed this through PlasFlow as well. But the size of my plasmids that i determined through PFGE is 30 kb and 25 kb respectively while the plasmid ...
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