User: gg

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Posts by gg

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Comment: C: Transform log2 fold changes in z-scores
... At first, the read counts were transformed in log2 values. The fold change decrease between treated and untreated control samples was calculated as described in the Equation below: gRNA C_score=[log_2⁡(gRNA abundance treated sample)-log_2⁡(gRNA abundance non treated sample) ] As each gene was targe ...
written 4 weeks ago by gg0
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Comment: C: Transform log2 fold changes in z-scores
... Hello, thanks both again for helping me. When I use FC values the plot looks like this (see below), which I find much more difficult to interpret, especially when I need to plot vertical lines to indicate FC-based thresholds. My aim is to compare two different approaches of analysis. In the specifi ...
written 4 weeks ago by gg0
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Comment: C: Transform log2 fold changes in z-scores
written 4 weeks ago by gg0 • updated 4 weeks ago by RamRS24k
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Comment: C: Transform log2 fold changes in z-scores
... Indeed that's what I did, does not the function scale() transform the data in z-scores? I still wonder if this is statistically correct... ...
written 4 weeks ago by gg0
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Transform log2 fold changes in z-scores
... Hello everyone! I have a dataset consisting of CRISPR gRNA read counts coming from two different samples (something very similar to a RNA-seq experiment output). I have transformed the read counts in log2 values and computed fold change between treated and non-treated sample. The distribution of ...
rna-seq written 4 weeks ago by gg0 • updated 4 weeks ago by The160

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