User: myrrlicht

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myrrlicht0
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Posts by myrrlicht

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Comment: C: Distinguish between homo- or heterozygote alleles if you sequence for a gene
... How should I know the differences in few nucleotides in the two alleles if this is what i want to find out? Meanwhile my direct sequencing of the exome of interest gave me two exact same height of amplitudes for two nucleotides... this seems to be proof enough to show heterogenity. ...
written 6 weeks ago by myrrlicht0
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Comment: C: Distinguish between homo- or heterozygote alleles if you sequence for a gene
... > 1 - Design PCR primers in such a way that you are reasonably sure to > tell apart the alleles you are interested in I think this is my problematic mental step i was asking for from the beginning...how is this possible if i have two copies of the gene/ allels on two chromosomes and i use the ...
written 8 weeks ago by myrrlicht0
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Comment: C: Distinguish between homo- or heterozygote alleles if you sequence for a gene
... Thanks for the answer! And: sorry, I assumed that everyone is sequencing by a capillary electrophoresis sequencing based method if it is Sanger or NGS anyway and this would not make a difference. So, if I amplify the gene and the sample would be homozygote i get one (in a simplified assumption) spec ...
written 8 weeks ago by myrrlicht0
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Distinguish between homo- or heterozygote alleles if you sequence for a gene
... How do you distinguish between homo- or heterozygote alleles if you sequence directly for a gene from genomic DNA? ...
sequencing written 8 weeks ago by myrrlicht0 • updated 8 weeks ago by Anima Mundi2.5k

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