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Showing :
questions
2
votes
4
replies
395
views
Should I exclude unmapped reads and proceed with the analysis for low mapped samples, or omit them altogether?
STAR
mapping
RNAseq
updated 10 weeks ago by
dthorbur
★ 1.9k • written 10 weeks ago by
Sib
▴ 60
0
votes
2
replies
484
views
Is there anything we can do when we have assignment rate below 50% in featurecounts?
RNA-seq
featurecounts
updated 10 months ago by
Ram
43k • written 10 months ago by
Sib
▴ 60
0
votes
0
replies
358
views
How are the reads assigned to genes in strand-specific RNAseq libraries?
strand-specific
strandness
RNAseq
10 months ago by
Sib
▴ 60
1
vote
3
replies
744
views
What is the benefit of knowing the source of contamination when we have RNAseq data with less than 70% mapping?
alignment
mapping
STAR
contamination
RNAseq
10 months ago by
Sib
▴ 60
2
votes
3
replies
884
views
Low mapping percentage
mapping
STAR
RNAseq
10 months ago by
Sib
▴ 60
1
vote
2
replies
788
views
Why my application works well but when I try to modularize it, it doesn't work properly ?
Shinymodule
app
Shinyapp
Shiny
module
17 months ago by
Sib
▴ 60
6
votes
7
replies
2.1k
views
How to understand logics and statistical concepts behind limma package?
R
microarray
DGE
limma
updated 2.5 years ago by
GenoMax
141k • written 2.5 years ago by
Sib
▴ 60
0
votes
0
replies
508
views
Using machine learning methods to find a biomarker panel to diagnose a disease.
Machine-learning
feature-selection
training-dataset
Biomarker
RNAseq
2.6 years ago by
Sib
▴ 60
0
votes
0
replies
867
views
Error in ReadAffy function
ReadAffy
Microarray
R
2.7 years ago by
Sib
▴ 60
3
votes
4
replies
3.8k
views
Error in ComBat function: Error in dat[, batch == batch_level] : (subscript) logical subscript too long
Batch-effect
microarray
R
updated 6 days ago by
Ram
43k • written 2.8 years ago by
Sib
▴ 60
0
votes
2
replies
810
views
Is it correct to merge two raw CEL datasets and then perform quality control, background correction, and normalization on it?
R
microarray
affymetrix
batch-effect
quality-control
updated 6 days ago by
Ram
43k • written 2.9 years ago by
Sib
▴ 60
1
vote
11
replies
2.6k
views
Error in volcano plot drawing with R.
R
updated 3.5 years ago by
Gordon Smyth
★ 7.0k • written 4.3 years ago by
Sib
▴ 60
4
votes
3
replies
1.3k
views
Can anyone introduce me a free tool for finding upstream regulators of differentially expressed genes?
upstream regulators
enrichment analysis
RNA-Seq
3.6 years ago by
Sib
▴ 60
0
votes
2
replies
6.7k
views
How can I put the Rtools on PATH?
R
updated 3.9 years ago by
ATpoint
81k • written 3.9 years ago by
Sib
▴ 60
0
votes
5
replies
1.3k
views
Is there a need for background correction and substitution of duplicates with the mean when we use the getGEO function of R in microarray analysis?
R
GEO
Microarray analysis
4.3 years ago by
Sib
▴ 60
7
votes
5
replies
2.1k
views
In R scripts of GEO2R which line is responsible for background correction and replacing replicated probes with the mean?
R
Differential expression analysis
4.3 years ago by
Sib
▴ 60
2
votes
4
replies
3.1k
views
Error in plotting Enhanced Volcano plot
differential expression analysis
enhanced volcano
updated 4.3 years ago by
dsull
★ 5.8k • written 4.3 years ago by
Sib
▴ 60
3
votes
4
replies
1.3k
views
After differential expression analysis if we use gene symbols for further analysis we lose some DEGs. Is it ok?
Differential expression analysis
GEO
4.3 years ago by
Sib
▴ 60
2
votes
2
replies
1.1k
views
String db knows some gene symbols of DEGs for more than one protein and after entering gene symbols ask to select the desiered gene.
R
Cytoscape
String app
network visualization
updated 4.3 years ago by
scooter
▴ 620 • written 4.4 years ago by
Sib
▴ 60
3
votes
1
reply
2.0k
views
Differentially expressed genes visualization with Cytoscape
Cytoscape
PPI network
visualization
updated 4.3 years ago by
padwalmk
▴ 140 • written 4.3 years ago by
Sib
▴ 60
2
votes
9
replies
4.5k
views
What this error means?Error in getEAWP(object) : data object isn't of a recognized data class
software error
R
4.4 years ago by
Sib
▴ 60
1
vote
2
replies
1.1k
views
which ID of differentially expressed genes from the result page of the GEO2R is used to the construction of the Protein-Protein interaction network i…
gene
4.4 years ago by
Sib
▴ 60
3
votes
6
replies
4.4k
views
How to obtain gene symbols from the result list of GEO2R?
rna-seq
R
updated 4.4 years ago by
prince26121991
▴ 70 • written 4.4 years ago by
Sib
▴ 60
7
votes
6
replies
2.0k
views
Are gene symbol and HGNC symbol the same names for a gene?
gene
RNA-Seq
updated 4.4 years ago by
dsull
★ 5.8k • written 4.4 years ago by
Sib
▴ 60
5
votes
7
replies
5.6k
views
R does not put the chart in pdf
R
updated 4.4 years ago by
Kevin Blighe
87k • written 4.4 years ago by
Sib
▴ 60
25 results • Page
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