You can try out reduce function
GRanges object with 1 range and 0 metadata columns:
seqnames ranges strand
 Chr1 2-30 +
seqinfo: 1 sequence from an unspecified genome; no seqlengths
... The gff3 file you are passing doesn't have the 'gene_id' attribute. Please do check the file and see the additional options in htseq to pass the correct id.
Some of other posts here that you can refer to:
and many more are th ...
**Registration Link:** : https://www.strand-ngs.com/webinar_registration
This webinar will focus on the gene fusion detection and characterization using Strand NGS, a sequencing data analysis software designed by biologists - for biologists.
Fusion events are commonly observed i ...
... You can check out https://gtexportal.org/home/ also for the normal sample tissues you are looking for. But not sure if it will have data regarding methylation. ...
... If you are comfortable in R, you can use the following code. Note: You will have to install the package seqinr before trying this
f <- read.fasta("input.fa")
Here are some of the similar posts you can refer to
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